Chanjing Feng#, Kaiyun Xin#, Yanfei Du#, Jingwen Zou, Xiaoxing Xing,Qi Xiu, Yijie Zhang, Rui Zhang, Weiwei Huang, Qinhu Wang , Cong Jiang ,Xiaojie Wang , Zhensheng Kang, Jin-Rong Xu , Huiquan Liu* . Unveiling the A-to-ImRNA editing machineryand its regulation and evolution in fungi, Nature Communications, 2024,15:3934.

Abstract

A-to-I mRNA editing in animals is mediated by ADARs, but the mechanism underlying sexual stage-specific A-to-I mRNA editing in fungi remains unknown. Here, we show that the eukaryotic tRNA-specific heterodimeric deaminase FgTad2-FgTad3 is responsible for A-to-I mRNA editing in Fusarium graminearum. This editing capacity relies on the interaction between FgTad3 and a sexual stage-specific protein called Ame1. Although Ame1 orthologs are widely distributed in fungi, the interaction originates in Sordariomycetes. We have identified key residues responsible for the FgTad3-Ame1 interaction. The expression and activity of FgTad2-FgTad3 are regulated through alternative promoters, alternative translation initiation, and post-translational modifications. Our study demonstrates that the FgTad2-FgTad3-Ame1 complex can efficiently edit mRNA in yeasts, bacteria, and human cells, with important implications for the development of base editors in therapy and agriculture. Overall, this study uncovers mechanisms, regulation, and evolution of RNA editing in fungi, highlighting the role of protein-protein interactions in modulating deaminase function.

https://www.nature.com/articles/s41467-024-48336-8?utm_campaign=related_content&utm_source=HEALTH&utm_medium=communities