study of inheritance and linkage of virulence genes in a selfing population of a Pakistani dominant race of Puccinia striiformis

論文信息: study of inheritance and linkage of virulence genes in a selfing population of a Pakistani dominant race of   Puccinia striiformis  f. sp.  tritici  . Sajid Mehmood, Marina Sajid, Syed Kamil Husnain, Jie Zhao*, Lili Huang*, and Zhensheng Kang. International Journal of Molecular Science.2020, 21, 1685. doi:10.3390/ijms21051685.

JCR分區(qū)Q1，中科院大類分區(qū)2區(qū)，IF=4.183

論文摘要： Wheat stripe rust is a severe threat of almost all wheat-growing regions in the world. Being an obligate biotrophic fungus,   Puccinia striiformis  f. sp.  tritici  ( PST ) produces new virulent races that break the resistance of wheat varieties. In this study, 115 progeny isolates were generated through sexual reproduction on susceptible Himalayan   Berberis pseudumbellata using a Pakistani dominant race (574232) of   PST . The parental isolate and progeny isolates were characterized using 24 wheat   Yr  single-gene lines and ten simple sequence repeat (SSR) markers. From the one-hundred-and-fifteen progeny isolates, 25 virulence phenotypes (VPs) and 60 multilocus genotypes were identified. The parental and all progeny isolates were avirulent to  Yr5 ,  Yr10 ,  Yr15 ,  Yr24 ,  Yr32 ,  Yr43 ,  YrSp ,  YrTr1 ,  YrExp2 ,  Yr26 , and   YrTye  and virulent to   Yr1 ,   Yr2  ,   Yr6 ,  Yr7 ,  Yr8 ,  Yr9 ,  Yr17 ,  Yr25 ,  Yr27 ,  Yr28 ,  YrA ,  Yr44 , and   Yr3 . Based on the avirulence/virulence phenotypes, we found that VPs virulent to   Yr1 ,  Yr2 ,  Yr9 ,  Yr17 ,  Yr47 , and   YrA were controlled by one dominant gene; those to YrSp ,  YrTr1 , and   Yr10  by two dominant genes; and those to   YrExp2 by two complementary dominant genes. The results are useful in breeding stripe rust-resistant wheat varieties and understanding virulence diversity.