A Novel MADS-box Transcription Factor PstMCM1-1 is Responsible for Full Virulence of Puccinia striiformis f. sp. Tritici

論文信息:Xiaoguo Zhu, Min Jiao, Jia Guo, Peng Liu, Chenglong Tan, Qian Yang, Yang Zhang, Ralf Thomas Voegele, Zhensheng Kang, Jun Guo. A Novel MADS-box Transcription Factor PstMCM1-1 is Responsible for Full Virulence of Puccinia striiformis f. sp. Tritici. Environmental Microbiology. DOI: 10.1111/1462-2920.14054

JCR分區(qū)Q1，IF=5.395

論文摘要： In many eukaryotes, transcription factor MCM1 gene plays crucial roles in regulating mating processes and pathogenesis by interacting with other co-factors. However, little is known about the role of MCM1 in rust fungi. Here, we identified two MCM1 orthologs, PstMCM1-1 and PstMCM1-2, in the stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst). Sequence analysis indicated that both PstMCM1-1 and PstMCM1-2 contain conserved MADS domains and that PstMCM1-1 belongs to a group of SRF-like proteins that are evolutionarily specific to rust fungi. Yeast two-hybrid assays indicated that PstMCM1-1 interacts with transcription factors PstSTE12 and PstbE1. PstMCM1-1 was found to be highly induced during early infection stages in wheat and during pycniospore formation on the alternate host barberry (Berberis shensiana). PstMCM1-1 could complement the lethal phenotype and mating defects in a mcm1 mutant of Saccharomyces cerevisiae. In addition, it partially complemented the defects in appressorium formation and plant infection in a Magnaporthe oryzae Momcm1 mutant. Knock down of PstMCM1-1 resulted in a significant reduction of hyphal extension and haustorium formation and the virulence of Pst on wheat. Our results suggest that PstMCM1-1 plays important roles in the regulation of mating and pathogenesis of Pst most likely by interacting with co-factors.